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Rabbit anti-NUDT6 Antibody

Product name: Rabbit anti-NUDT6 Antibody
Catalog:

DL97198A

Synonyms : FGF2AS; Nucleoside diphosphate-linked moiety X motif 6; Nudix motif 6; Antisense basic fibroblast growth factor; Protein GFG
Reactivity: H, M, R
Applications: WB, IHC, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: Recombinant full length protein of human NUDT6
WB description: Western blot analysis of NUDT6 expression in Jurkat (A), HepG2 (B) whole cell lysates.
IHC description: Immunohistochemical analysis of NUDT6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:

Immunofluorescent analysis of NUDT6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Gene ID(Human): 11162
Gene ID (Mouse)): 229228
Gene ID(Rat): 207120
SwissProt (Human): P53370
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.