地址：江苏省无锡市梁溪区锡沪东路8号301-2 电话：0510-82732223 / 86-0510-82732223 邮箱：info@dldevelop.com.cn
备案号：苏ICP备06050612号-1 苏公网安备 32020302000048号 版权所有 @2016 无锡市东林科技发展有限责任公司Language:English
电话:86-0510-82732223
邮箱:info@dldevelop.com.cn
| Product name: | Rabbit anti-SFRP4 Antibody |
| Catalog: | DL97092A |
| Synonyms : | FRPHE; Secreted frizzled-related protein 4; sFRP-4; Frizzled protein, human endometrium; FrpHE |
| Reactivity: | H, M, R |
| Applications: | WB, IHC, IF/IC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | Recombinant full length protein of human SFRP4 |
| WB description: | Western blot analysis of SFRP4 expression in HepG2 (A), Hela (B) whole cell lysates. |
| IHC description: | Immunohistochemical analysis of SFRP4 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description: | Immunofluorescent analysis of SFRP4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100) |
| Gene ID(Human): | 6424 |
| Gene ID (Mouse)): | 20379 |
| Gene ID(Rat): | 89803 |
| SwissProt (Human): | Q6FHJ7 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |