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| Product name: | Rabbit anti-eIF3E Antibody |
| Catalog: | DL96075A |
| Synonyms : | EIF3S6; INT6; Eukaryotic translation initiation factor 3 subunit E; eIF3e; Eukaryotic translation initiation factor 3 subunit 6; Viral integration site protein INT-6 homolog; eIF-3 p48 |
| Reactivity: | H, M, R |
| Applications: | WB, IF/IC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | Recombinant full length protein of human eIF3E |
| WB description: | Western blot analysis of eIF3E expression in MCF7 (A), K562 (B), HepG2 (C) whole cell lysates. |
| IF/ICC description: | Immunofluorescent analysis of eIF3E staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/2000), IF/IC (1/50 - 1/100) |
| Gene ID(Human): | 3646 |
| Gene ID (Mouse)): | 16341 |
| Gene ID(Rat): | 299872 |
| SwissProt (Human): | P60228 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |