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| Product name: | Rabbit anti-eIF4A1 Antibody |
| Catalog: | DL96043A |
| Synonyms : | DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1 |
| Reactivity: | H, M, R |
| Applications: | WB, IF/IC, IP |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | Recombinant full length protein of human eIF4A1 |
| WB description: | Western blot analysis of eIF4A1 expression in Hela (A), Jurkat (B), NIH3T3 (C), PC12 (D), mouse liver (E) whole cell lysates. |
| IF/ICC description: | Immunofluorescent analysis of eIF4A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50) |
| Gene ID(Human): | 1973 |
| Gene ID (Mouse)): | 13681 |
| SwissProt (Human): | P60842 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |