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Rabbit anti-MTAP Antibody

Product name: Rabbit anti-MTAP Antibody
Catalog:

DL95883A

Synonyms : MSAP; S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; MTAPase
Reactivity: H, M, R
Applications: WB, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: Recombinant full length protein of human MTAP
WB description: Western blot analysis of MTAP expression in 22RV1 (A), HEK293T (B), mouse liver (C) whole cell lysates.
IF/ICC description:

Immunofluorescent analysis of MTAP staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Gene ID(Human): 4507
Gene ID (Mouse)): 66902
SwissProt (Human): Q13126
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.