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| Product name: | Rabbit anti-c-RAF Antibody |
| Catalog: | DL95611A |
| Synonyms : | RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1 |
| Reactivity: | H |
| Applications: | WB, IHC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | Recombinant protein corresponding to human c-RAF. |
| WB description: | Western blot analysis of c-RAF expression in Hela (A) whole cell lysates. |
| IHC description: | Immunohistochemical analysis of c-RAF staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/1000 - 1/2000), IH (1/50 - 1/200) |
| Gene ID(Human): | 5894 |
| SwissProt (Human): | P04049 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |