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| Product name: | Mouse anti-SMAD3 Antibody |
| Catalog: | DL95549A |
| Synonyms : | MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 |
| Reactivity: | H, M, R |
| Applications: | WB, IHC |
| Size: | 50uL/100uL |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Concentration: | 1mg/mL |
| Immunogen: | Recombinant protein corresponding to human SMAD3. |
| WB description: | Western blot analysis of SMAD3 expression in 293T (A), Jurkat (B), mouse heart (C), rat heart (D) whole cell lysates. |
| IHC description: | Immunohistochemical analysis of SMAD3 staining in human liver,human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| Purification_Method: | |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/1000 - 1/2000), IH (1/100 - 1/200) |
| Gene ID(Human): | 4088 |
| SwissProt (Human): | P84022 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |