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Mouse anti-NF-kappaB p65 Antibody

Product name: Mouse anti-NF-kappaB p65 Antibody
Catalog:

DL95538A

Synonyms : NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Reactivity: H, M, R
Applications: WB, IHC, IP
Size: 50uL/100uL
Host: Mouse
Clonality: Monoclonal
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human NF-kappaB p65. The exact sequence is proprietary.
WB description: Western blot analysis of NF-kappaB p65 expression in Hela (A), 293T (B), HepG (C) whole cell lysates.
IHC description: Immunohistochemical analysis of NF-kappaB p65 staining in mouse hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IP description: Immunoprecipitation of NF-kappaB p65 from 0.5mg Hela whole cell extract lysate, using anti-NF-kappaB p65 Antibody.
Purification_Method:
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/1000 - 1/3000), IH (1/100 - 1/200), IP (1/50 - 1/200)
Gene ID(Human): 5970
Gene ID (Mouse)): 19697
SwissProt (Human): Q04206
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.