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| Product name: | Mouse anti-EGFR Antibody |
| Catalog: | DL95491A |
| Synonyms : | ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
| Reactivity: | H |
| Applications: | WB, IF/IC, IP |
| Size: | 50uL/100uL |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence of human EGFR. The exact sequence is proprietary. |
| WB description: | Western blot analysis of EGFR expression in A431 (A), Hela (B) whole cell lysates. |
| IF/ICC description: | Immunofluorescent analysis of EGFR staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
| IP description: | Immunoprecipitation of EGFR from 0.5mg Hela whole cell extract lysate, using anti-EGFR Antibody. |
| Purification_Method: | |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200), IP (1/100 - 1/200) |
| Gene ID(Human): | 1956 |
| SwissProt (Human): | P00533 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |