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| Product name: | Rabbit anti-HMGB1 Antibody |
| Catalog: | DL95073A |
| Synonyms : | HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1 |
| Reactivity: | H, M, R, B, C, D, Mk, P |
| Applications: | WB, IHC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HMGB1. The exact sequence is proprietary. |
| WB description: | Western blot analysis of HMGB1 expression in HepG2 UV-treated (A) whole cell lysates. |
| IHC description: | Immunohistochemical analysis of HMGB1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IH (1/100 - 1/200) |
| Gene ID(Human): | 3146 |
| Gene ID (Mouse)): | 100862258; 15289 |
| Gene ID(Rat): | 25459 |
| SwissProt (Human): | P09429 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |