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Product name: | Rabbit anti-AMPK gamma 1/2/3 Antibody |
Catalog: | DL94813A |
Synonyms : | PRKAG1; 5'-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK subunit gamma-1; AMPKg; PRKAG2; 5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma2; AMPK subunit gamma-2; H91620p; PRKAG3; AMPKG3; 5'-AMP-activated protein kinase subunit gamma-3; AMPK gamma3; AMPK subunit gamma-3 |
Reactivity: | H, M, R, B, P |
Applications: | WB, IF/IC |
Size: | 50uL/100uL |
Host: | Rabbit |
Clonality: | Polyclonal |
Concentration: | 1mg/mL |
Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AMPK gamma 1/2/3. The exact sequence is proprietary. |
WB description: | Western blot analysis of AMPK gamma 1/2/3 expression in HEK293T (A), HepG2 (B), MCF7 (C), PC3 (D) whole cell lysates. |
IF/ICC description: | Immunofluorescent analysis of AMPK gamma 1/2/3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Dilution: | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
Gene ID(Human): | 5571; 51422; 53632; |
Gene ID (Mouse)): | 19082; 108099; 241113 |
Gene ID(Rat): | 25520 |
SwissProt (Human): | P54619; Q9UGJ0; Q9UGI9 |
Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |