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Product name: | Rabbit anti-HIF1 beta Antibody |
Catalog: | DL93335A |
Synonyms : | BHLHE2; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta |
Reactivity: | H, R, B, P, Rb |
Applications: | WB, IHC |
Size: | 50uL/100uL |
Host: | Rabbit |
Clonality: | Polyclonal |
Concentration: | 1mg/mL |
Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HIF1 beta. The exact sequence is proprietary. |
WB description: | Western blot analysis of HIF1 beta expression in HeLa (A), U2OS (B), rat muscle (C) whole cell lysates. |
IHC description: | Immunohistochemical analysis of HIF1 beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Dilution: | WB (1/500 - 1/1000), IH (1/100 - 1/200) |
Gene ID(Human): | 405 |
Gene ID (Mouse)): | 11863 |
Gene ID(Rat): | 25242 |
SwissProt (Human): | P27540 |
Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |