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| Product name: | Rabbit anti-GCLM Antibody |
| Catalog: | DL92959A |
| Synonyms : | GLCLR; Glutamate--cysteine ligase regulatory subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit |
| Reactivity: | H, M, R, B |
| Applications: | WB, IHC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GCLM. The exact sequence is proprietary. |
| WB description: | Western blot analysis of GCLM expression in SHSY5Y (A), HeLa (B), 3T3 (C) whole cell lysates. |
| IHC description: | Immunohistochemical analysis of GCLM staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IH (1/100 - 1/200) |
| Gene ID(Human): | 2730 |
| Gene ID (Mouse)): | 14630 |
| Gene ID(Rat): | 29739 |
| SwissProt (Human): | P48507 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |