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| Product name: | Rabbit anti-Cyclin E1 (pT395) Antibody |
| Catalog: | DL92161A |
| Synonyms : | CCNE; G1/S-specific cyclin-E1 |
| Reactivity: | H |
| Applications: | WB, IHC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cyclin E1 (pT395). The exact sequence is proprietary. |
| WB description: | Western blot analysis of Cyclin E1 (pT395) expression in HepG2 (A), JAR (B) whole cell lysates. |
| IHC description: | Immunohistochemical analysis of Cyclin E1 (pT395) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IH (1/100 - 1/200) |
| Gene ID(Human): | 898 |
| SwissProt (Human): | P24864 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |