地址：江苏省无锡市梁溪区锡沪东路8号301-2 电话：0510-82732223 / 86-0510-82732223 邮箱：info@dldevelop.com.cn
备案号：苏ICP备06050612号-1 苏公网安备 32020302000048号 版权所有 @2016 无锡市东林科技发展有限责任公司Language:English
电话:86-0510-82732223
邮箱:info@dldevelop.com.cn
| Product name: | Rabbit anti-p39 Antibody |
| Catalog: | DL91338A |
| Synonyms : | NCK5AI; Cyclin-dependent kinase 5 activator 2; CDK5 activator 2; Cyclin-dependent kinase 5 regulatory subunit 2; p39; p39I |
| Reactivity: | H |
| Applications: | WB, IHC, IF/IC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p39. The exact sequence is proprietary. |
| WB description: | Western blot analysis of p39 expression in SHSY5Y (A), HeLa (B), U87MG (C) whole cell lysates. |
| IHC description: | Immunohistochemical analysis of p39 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description: | Immunofluorescent analysis of p39 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) |
| Gene ID(Human): | 8941 |
| SwissProt (Human): | Q13319 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |