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| Product name: | Rabbit anti-AMPK beta 1 (pS182) Antibody |
| Catalog: | DL90926A |
| Synonyms : | AMPK; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb |
| Reactivity: | H, M, R, B, P |
| Applications: | WB, IHC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AMPK beta 1. The exact sequence is proprietary. |
| WB description: | Western blot analysis of AMPK beta 1 (pS182) expression in A431 (A), NIH3T3 (B), PC12 (C) whole cell lysates. |
| IHC description: | Immunohistochemical analysis of AMPK beta 1 (pS182) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IH (1/100 - 1/200) |
| Gene ID(Human): | 5564 |
| Gene ID (Mouse)): | 19079 |
| Gene ID(Rat): | 83803 |
| SwissProt (Human): | Q9Y478 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |