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| Product name: | Rabbit anti-PDGFR alpha Antibody |
| Catalog: | DL90866A |
| Synonyms : | PDGFR2; RHEPDGFRA; Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2; CD140a |
| Reactivity: | H, M, R, B, C, D, Mk, P |
| Applications: | WB, IF/IC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PDGFR alpha. The exact sequence is proprietary. |
| WB description: | Western blot analysis of PDGFR alpha expression in HepG2 (A), NIH3T3 (B), Saos2 (C), HeLa (D), A431 (E) whole cell lysates. |
| IF/ICC description: | Immunofluorescent analysis of PDGFR alpha staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
| Gene ID(Human): | 5156 |
| Gene ID (Mouse)): | 18595 |
| Gene ID(Rat): | 25267 |
| SwissProt (Human): | P16234 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |