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| Product name: | Rabbit anti-MLH1 Antibody |
| Catalog: | DL90742A |
| Synonyms : | COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1 |
| Reactivity: | H |
| Applications: | WB, IF/IC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MLH1. The exact sequence is proprietary. |
| WB description: | Western blot analysis of MLH1 expression in KNRK (A), HeLa (B), A431 (C), SW480 (D) whole cell lysates. |
| IF/ICC description: | Immunofluorescent analysis of MLH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
| Gene ID(Human): | 4292 |
| SwissProt (Human): | P40692 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |