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| Product name: | Rabbit anti-Cytokeratin 7 Antibody |
| Catalog: | DL90657A |
| Synonyms : | SCL; Keratin type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7 |
| Reactivity: | H |
| Applications: | WB, IHC, IF/IC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 7. The exact sequence is proprietary. |
| WB description: | Western blot analysis of Cytokeratin 7 expression in HeLa (A), A431 (B) whole cell lysates. |
| IHC description: | |
| IF/ICC description: | Immunofluorescent analysis of Cytokeratin 7 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) |
| Gene ID(Human): | 3855 |
| SwissProt (Human): | P08729 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |