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| Product name: | Rabbit anti-CD3d Antibody |
| Catalog: | DL90177A |
| Synonyms : | T3D; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD3d |
| Reactivity: | H, Mk |
| Applications: | WB, IF/IC |
| Size: | 50uL/100uL |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Concentration: | 1mg/mL |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD3d. The exact sequence is proprietary. |
| WB description: | Western blot analysis of CD3d expression in CCRFCEM (A), Jurkat (B), HuT78 (C) whole cell lysates. |
| IF/ICC description: | Immunofluorescent analysis of CD3d staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution: | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
| Gene ID(Human): | 915 |
| SwissProt (Human): | P04234 |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |